Hydroxypropyl Cyclodextrin Improves Amiodarone-induced Aberrant Lipid Homeostasis of Alveolar Cells

Alveolar epithelial type II (AT2) cells secrete pulmonary surfactant via lamellar bodies (LBs). Abnormalities in LBs are associated with disorders, including fibrosis. However, high-content screening (HCS) for LB abnormalities is limited by the lack of understanding AT2 cell functions. In present study, we have developed harboring LB-like organelles that proteins. These were more similar to than parental A549 cells. recapitulated amiodarone (AMD)-induced enlargement, patients exposed AMD. To reverse AMD-induced abnormalities, performed HCS approved drugs and identified 2-hydroxypropyl-?-cyclodextrin (HP?CD), a cyclic oligosaccharide, as potential therapeutic agent. A transcriptome analysis revealed HP?CD modulates lipid homeostasis. addition, inhibited human induced pluripotent stem cell–derived Our results demonstrate useful suggest candidate agent interstitial pneumonia.